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9820-21 Fastidious Gram-Negative Bacilli
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FREE FOR MEMBERS!
Course includes study material and exam.
Description: Learn about the classification and nomenclature, morphology and growth requirements, identification and susceptibility, pathogenicity and relationship to differentiation strategies for Haemophilus, Bordetella, Pasteurella, Capnocytophaga and Aggregatibacter, Eikenella, Kingella, and Cardiobacterium.
Start date: Upon registration
Completion: Up to 52 weeks
Credits awarded per Session. See individual Sessions for further details.
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About this item

FREE FOR MEMBERS!

Course includes study material and exam.

Description: Learn about the classification and nomenclature, morphology and growth requirements, identification and susceptibility, pathogenicity and relationship to differentiation strategies for Haemophilus, Bordetella, Pasteurella, Capnocytophaga and Aggregatibacter, Eikenella, Kingella, and Cardiobacterium.

Start date: Upon registration

Completion: Up to 52 weeks

Course/Activity Information

Learning Outcomes:

  • State the criteria originally used to place an organism in the genus

Haemophilus influenzae

  • Describe the cellular morphology.
  • Describe the growth requirements.
  • Describe colonial morphology on blood agar with a staph streak and on chocolate agar.
  • State the criteria necessary to establish the genus Haemophilus.
  • State how to differentiate the different species of Haemophilus using hemolysis, growth factor requirements and porphyrin test.
  • Explain why biotyping and serotyping would be carried out and state which serotype is the most virulent.
  • State the approximate rate of resistance to ampicillin, describe the two mechanisms responsible for the resistance and how each is detected.
  • Name a rapid test for chloramphenicol resistance.
  • Explain why childhood infections due to this organism are decreasing in number.
  • Name the two invasive infections found in children and describe typical findings in spinal fluid from a case of meningitis.

Haemophilus aegyptius

  • Explain the taxonomic relationship with influenzae.
  • Describe two acute infections caused by aegyptius.

Other Haemophilus species

  • Describe the clinical significance of parainfluenzae, H. haemolyticus and H. parahaemolyticus.
  • Describe the cellular morphology of ducreyi in direct smears and in cultures.
  • State the pathogenicity of ducreyi.

X and V Growth Factor Requirements

  • State the criteria the test medium must meet and name the recommended medium.
  • State how the test medium should be inoculated, how carryover of growth factors in the inoculum would affect results and how to avoid this.
  • Describe how to interpret results.

Porphyrin Test

  • State the principle of the test including the substrate used.
  • State how the test is inoculated and describe two methods of detecting porphyrins.
  • State how to interpret test results.
  • Explain how the test interpretation is affected by:
    • Testing bacteria belonging to another genus
    • Using non-viable bacteria
    • Using too light an inoculum

Bordetella

  • Name the species most commonly infecting man.

Bordetella pertussis

  • Describe typical cellular morphology.
  • Describe how diagnosis may be made using direct smears.
  • Name the site that should be sampled for culture and explain how the specimen is obtained.
  • Name and describe a suitable transport medium.
  • Describe the growth requirements.
  • Name two media used for isolation of pertussis, compare the shelf life and name the antibiotic recommended for these media.
  • State the usual time required for visible colonies to form.
  • State the criteria indicating the genus Bordetella.
  • State how to differentiate pertussis from B. parapertussis and B. bronchiseptica.
  • Name the disease caused by pertussis and describe the symptoms.
  • Describe the clinical significance of parapertussis and B. bronchiseptica.

Capnocytophaga

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate Capnocytophaga.
  • State how complete species identification is carried out.
  • Describe the pathogenicity of canimorsus and state the usual source of the bacteria.

Aggregatibacter

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate Aggregatibacter
  • Describe the pathogenicity of Aggregatibacter and state the usual source of the bacteria.

Eikenella

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate Eikenella
  • Describe the pathogenicity of Eikenella and state the usual source of the bacteria.

Kingella

  • Describe typical cellular morphology.
  • State suitable media and atmosphere for growth.
  • Describe typical colonies.
  • Describe laboratory findings that indicate
  • Describe the pathogenicity of Kingella and state the usual source of the bacteria.

Cardiobacterium

  • Describe typical cellular and colonial morphology.
  • Describe pathogenicity.

Pasteurella

  • Name the species most commonly isolated from man.
  • Name the genus to which some Pasteurella species are being moved.

Pasteurella multocida

  • Describe typical cellular morphology.
  • Describe growth requirements.
  • Describe typical colonies on blood agar.
  • State criteria that indicate a possible multocida. State the usual source of the bacteria infecting man and describe clinical infection.

 

Author: Helen Smith, MLT

Course Revisions: Erin Jansen, MLT

Version Date: July 2021

 

PEP hours: 11

CPS credits: 0.45

*Note: PEP hours and/or CPS credits will only be awarded upon successful completion of Final Exam.

 

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