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9815-22 Theory and Mechanisms of Histopathological Staining
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Description: Review the theory and mechanisms of staining, dye classification and tissue demonstration methods and improve your skills and the quality of prepared material.
Start date: Upon registration
Completion: Up to 52 weeks
Credits awarded per Session. See individual Sessions for further details.
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FREE FOR MEMBERS!

Description: Review the theory and mechanisms of staining, dye classification and tissue demonstration methods and improve your skills and the quality of prepared material.

Start date: Upon registration

Completion: Up to 52 weeks

 

Course/Activity Information

Prior to attempting this course, the learner will have:

  • Understanding of introductory microanatomy terminology
  • Ability to recognize microanatomical components of tissue smears and sections

Learning Outcomes:

  • Define the term resonance system in relation to dye chemistry
  • Define the following terms in relation to dye structure and classification:
  1. Chromophore (quinoid ring, azo coupling, nitro grouping)
  2. Chromogen
  3. Auxochrome (hydroxyl, amine, carboxyl, sulphonic)
  • Relate acid, basic and neutral dyes to their staining properties
  • Discuss physical theories of staining: selective solubility, adsorption, porosity
  • Describe chemical theories of staining: salt linkage, hydrogen bonding, metachromasia, leukostaining, metallic impregnation, histochemical reactions
  • Describe proper storage of dyes
  • Define colour index number
  • Differentiate between progressive and regressive staining and state advantages and disadvantages of each
  • Classify dyes according to origin and give examples of each
  • Describe and define accelerators and accentuators.
  • State principle and application of argyrophil and argentaffin / modified argentaffin reactions
  • Name three chromophoric groups found in many commonly used stains and show the basic chemical structure and name at least one dye that contains the group
  • Define:
  1. Basophilic tissue
  2. Acidophilic tissue
  • Define the specificity of the following staining mechanisms:
  1. Mordant
  2. Lake
  3. Accentuator
  4. Accelerator
  5. Metachromasia
  6. Leuco compound (also spelled leuko compound)
  7. Chromotrope
  8. Orthochromasia

 

Author: Masaye Tanaka, ART, BSc, MEd

Version Date: January 2022

 

PEP hours: 12.5

CPS credits: 0

*Note: PEP hours and/or CPS credits will only be awarded upon successful completion of Final Exam.

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