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FREE FOR MEMBERS!
Course includes study material and exam.
Description: Learn how to categorize different types of fixing agents; to elaborate on concepts in fixation and preservation of cellular elements for different types of analysis; and to utilize principles of tissue/cell preservation and fixation in order to effectively demonstrate specific components of cells and tissues.
Start Date: Upon registration
Completion: Up to 52 weeks
Learning Outcomes:
o Identify chemical structure of acetone, formaldehyde, ethanol, formic acid, picric acid, methanol, acetic acid, mercuric chloride, glutaraldehyde, osmium tetroxide, potassium dichromate, glyoxal
o Describe features of fixing agents:
o B-Plus™ (zinc based fixatives)
o Bouin's, Hollande
o Ethanol and alcohol solutions
o Formaldehyde, NBF
o Glutaraldehyde
o Michel transport medium
o Zenker, Helly, B-5 and mercury-based fixatives
o Proprietary reagents
PreservcytTM
CytolytTM
IBFTM
-AZFTM
o Formaldehyde
o Ethanol and alcohol solutions
o Picric acid
o Osmium tetroxide
o Mercuric chloride
o Zinc compounds
o Tolerant fixative
o Intolerant fixative
o Simple fixative
o Compound fixative
o Coagulant fixative
o Non-coagulant fixative
o Additive fixative
o Non-additive fixative
o Use of personal protective equipment (PPE)
o Exposure limits (OEL)
Author: Masaye Tanaka, ART, BSc, MEd
Version Date: October 2016
PEP hours: 10
CPS credits: 0
*Note: PEP hours and/or CPS credits will only be awarded upon successful completion of Final Exam.
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