Catalog

9893-18 Fixation Practices
Online
Added:

FREE FOR MEMBERS!
Course includes study material and exam.
Description: Learn how to categorize different types of fixing agents; to elaborate on concepts in fixation and preservation of cellular elements for different types of analysis; and to utilize principles of tissue/cell preservation and fixation in order to effectively demonstrate specific components of cells and tissues.
Start Date: Upon registration
Completion: Up to 52 weeks
Credits awarded per Session. See individual Sessions for further details.
Credits awarded
Type
Name
Sub type
Value
Certificate awarded

Recently Added Items

    There's nothing to show just yet.

About this item

FREE FOR MEMBERS!

Course includes study material and exam.

Description: Learn how to categorize different types of fixing agents; to elaborate on concepts in fixation and preservation of cellular elements for different types of analysis; and to utilize principles of tissue/cell preservation and fixation in order to effectively demonstrate specific components of cells and tissues. 

Start Date: Upon registration

Completion: Up to 52 weeks

Course/Activity Information

Learning Outcomes:

  • Describe basic structure and reactions of protein in relation to fixation
  • Define purpose/function of fixation

o   Identify chemical structure of acetone, formaldehyde, ethanol, formic acid, picric acid, methanol, acetic acid, mercuric chloride, glutaraldehyde, osmium tetroxide, potassium dichromate, glyoxal

o   Describe features of fixing agents:

o   B-Plus™ (zinc based fixatives)

o   Bouin's, Hollande

o   Ethanol and alcohol solutions

o   Formaldehyde, NBF

o   Glutaraldehyde

o   Michel transport medium

o   Zenker, Helly, B-5 and mercury-based fixatives

o   Proprietary reagents

PreservcytTM

CytolytTM

IBFTM

-AZFTM

  • Define preservation and differentiate from fixation.
  • Identify fixation/fixative artifacts
  • Describe the effects and optimization of temperature, volume of fixative, osmolality, concentration, time of exposure and [H+] concentration on fixation of tissue
  • Describe how the size of tissue affects fixation
  • State the optimal thickness of tissue for paraffin processing for light microscopy
  • State the optimal thickness of tissue for electron microscopy
  • Differentiate between non-coagulant and coagulant fixative
  • Describe the mode of action and general classification, advantages, disadvantages and safety precautions associated with:

o   Formaldehyde

o   Ethanol and alcohol solutions

o   Picric acid

o   Osmium tetroxide

o   Mercuric chloride

o   Zinc compounds

  • Define

o   Tolerant fixative

o   Intolerant fixative

o   Simple fixative

o   Compound fixative

o   Coagulant fixative

o   Non-coagulant fixative

o   Additive fixative

o   Non-additive fixative

  • Define autolysis: cytolysis, karyorrhexis, karyolysis
  • Define putrefaction
  • Describe conditions/analyses when/where fresh, unfixed tissue is required
  • Define/Describe the use of post-fixation
  • Discuss the theories regarding mechanisms of various types of fixatives
  • Describe handling, disposal and storage of reagents used for fixatives

o   Use of personal protective equipment (PPE)

o   Exposure limits (OEL)

 

Author: Masaye Tanaka,  ART, BSc, MEd

Version Date: October 2016

 

PEP hours: 10

CPS credits: 0

*Note: PEP hours and/or CPS credits will only be awarded upon successful completion of Final Exam.

Close
Shopping Cart
Total:  Close

Loading Shopping Cart Items...